Coding

Part:BBa_K5007002

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-03)

C. Sativa Hexanoyl-CoA Synthase without stop-codon.

This part codes for Cannabis sativa’s acyl-activating enzyme 1, or Hexanoyl-CoA synthase, responsible for the activation of hexanoate in the first step for the synthesis of olivetolic acid. This part has the stop-codon removed for fusion into monocystronic composite parts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 228
    Illegal NgoMIV site found at 1531
    Illegal AgeI site found at 1090
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 771


References

Stout JM, Boubakir Z, Ambrose SJ, Purves RW, Page JE. The hexanoyl-CoA precursor for cannabinoid biosynthesis is formed by an acyl-activating enzyme in Cannabis sativa trichomes. Plant J. 2012 Aug;71(3):353-65. doi: 10.1111/j.1365-313X.2012.04949.x. Epub 2012 Jun 1. PMID: 22353623.


[edit]
Categories
Parameters
None